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Download scientific diagram | MultiSite Gateway system. In the MultiSite Gateway system, att 1, att 2, att 3 and att 4 sequences are used for cloning of multiple DNA fragments into one vector. A promoter entry clone (L4-Pro-R1), ORF entry clone (L1-ORF-L2), tag entry clone (R2-tag-L3) and destination vector R4-R3 are subjected to an LR reaction. The promoter, ORF and tag sequences are linked and incorporated into the destination vector to form a promoter:ORF- tag clone. B1 , att B1; B2 , att B2; B3 , att B3; B4 , att B4; L1 , att L1; L2 , att L2; L3 , att L3; L4 , att L4; R1 , att R1; R2 , att R2; R3 , att R3; R4 , att R4; P1 , att P1; P2 , att P2; P3 , att P3; P4 , att P4; P1R , att P1R; P2R ; att P2R; Cm r , chloramphenicol-resistance marker; ccd B, negative selection marker in from publication: Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction | Plant Genetic Engineering, BiFC and Complement
Valuable clone collections encoding the complete ORFeomes for some model organisms have been constructed following the completion of their genome sequencing projects. These libraries are based on Gateway cloning technology, which facilitates the study …